Review




Structured Review

Schuelke tailed primer method
Tailed Primer Method, supplied by Schuelke, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+method/tailed+primer+method/pmc11785656-117-7-10
Average 90 stars, based on 1 article reviews
tailed primer method - by Bioz Stars, 2026-10
90/100 stars

Images

Related Articles

other:

Article Title: Inter-island differentiation and contrasting patterns of diversity in the iconic Canary Island sub-alpine endemic Echium wildpretii (Boraginaceae)
Article Snippet: Inter-island differentiation and contrasting patterns of diversity in the iconic Canary Island sub-alpine endemic Echium wildpretii (Boraginaceae) Rachael E. Graham, J. Alfredo Reyes-Betancort, Mark A. Chapman & Mark A. Carine To cite this article: Rachael E. Graham, J. Alfredo Reyes-Betancort, Mark A. Chapman & Mark A. Carine (2021): Inter-island differentiation and contrasting patterns of diversity in the iconic Canary Island sub-alpine endemic Echium wildpretii (Boraginaceae), Systematics and Biodiversity, DOI: 10.1080/14772000.2021.1877847 To link to this article: https://doi.org/10.1080/14772000.2021.1877847

Article Title: Global analysis of Hemileia vastatrix populations shows clonal reproduction for the coffee leaf rust pathogen throughout most of its range
Article Snippet: Hemileia vastatrix is the most important fungal pathogen of coffee and the causal agent of recurrent disease epidemics that have invaded nearly every coffee growing region in the world.. The development of coffee varieties resistant to H. vastatrix requires fundamental understanding of the biology of the fungus.. However, the complete life cycle of H. vastatrix remains unknown, and conflicting studies and interpretations exist as to whether the fungus is undergoing sexual reproduction.

Article Title: Genetic structure of <i>Plasmopara halstedii</i> populations across Europe and South Russia
Article Snippet: 1Department of Botany, Faculty of Science, Palacký University in Olomouc, Olomouc, Czech Republic 2Senckenberg Biodiversity and Climate Research Center, Senckenberg Gesellschaft für Naturforschung, Frankfurt am Main, Germany 3Department of Biology, Institute of Ecology, Evolution, and Diversity, Goethe University, Frankfurt am Main, Germany 4Translational Biodiversity Genomics Centre (TBG), Frankfurt am Main, Germany 5Department of Integrated Plant Protection, Institute of Plant Protection, Hungarian University of Agriculture and Life Sciences, Gödöllő, Hungary 6V.S.. Pustovoit AllRussian Research Institute of Oil Crops, Krasnodar, Russia 7Department of Cell Biology and Genetics, Faculty of Science, Palacký University in Olomouc, Olomouc, Czech Republic 8Institute of Biology, University of Hohenheim, Stuttgart, Germany

Article Title: Development and characterization of 33 polymorphic microsatellite markers in Trachurus japonicus by SLAF‐seq technology
Article Snippet: The Japanese jack mackerel, Trachurus japonicus (Temminck & Schlegel, 1844), is a common species belonging to family Carangidae (Perciformes), which inhabits the warm-temperate waters of the northwestern Pacific Ocean (Liu et al., 2016).. The species is also one of the most economically and ecologically important pelagic fish in China seas (Liu et al., 2016).. However, the annual catch of T. japonicus in China seas has experienced serious fluctuations in the past 20 years.

Article Title: Reproductive biology and population structure of the endangered shrub Grevillea bedggoodiana (Proteaceae)
Article Snippet: The plants were genotyped using the three primer method implementing four fluorescently labelled universal primers (Schuelke 2000; Blacket et al. 2012) and fourteen sets of primers designed to amplify microsatellite loci in two closely related species (Grevillea aquifolium and G. infecunda; James et al. 2012).

Amplification:

Article Title: Identifying Priority Giant Anteater ( Myrmecophaga tridactyla ) Populations for Conservation in São Paulo State, Brazil
Article Snippet: .. Amplification via PCR was performed with the addition of the M13 tail, which allowed for the use of the tailed primer method (Schuelke, ). .. The amplifications were performed using touchdown PCR in a total volume of 17.2 μl, which contained 1 μl of DNA (~50 ηg), 0.2 μl of the forward primer (5 μM), 0.4 μl of the M13 primer dyed with fluorophore (5 μ), 0.6 μl of the reverse primer (5 μM), 5 μl of the Gotaq ® Colorless Master Mix (Promega; 2X), and 10 μl of ultrapure water (Don et al., ; Korbie & Mattick, ).

Article Title: Genome-Assisted Gene-Flow Rescued Genetic Diversity Without Hindering Growth Performance in an Inbred Coho Salmon ( Oncorhynchus kisutch ) Population Selected for High Growth Phenotype
Article Snippet: Genomic DNA was collected from an adipose fin clip using a Gentra Puregene Tissue Kit (QIAGEN). .. The 11 loci were amplified using the tailed primer method (Schuelke ; Sekino et al. ) where 19-bp of the M13 sequence (CACGACGTTGTAAAACGAC) was added to the 5' end of the forward primer to label PCR amplicon with a fluorescent dye (i.e., FAM, VIC, NED, PED). .. Polymorphisms were analyzed on an ABI3130 DNA Analyzer (Applied Biosystems, MA, USA).

Polymerase Chain Reaction:

Article Title: Identifying Priority Giant Anteater ( Myrmecophaga tridactyla ) Populations for Conservation in São Paulo State, Brazil
Article Snippet: .. Amplification via PCR was performed with the addition of the M13 tail, which allowed for the use of the tailed primer method (Schuelke, ). .. The amplifications were performed using touchdown PCR in a total volume of 17.2 μl, which contained 1 μl of DNA (~50 ηg), 0.2 μl of the forward primer (5 μM), 0.4 μl of the M13 primer dyed with fluorophore (5 μ), 0.6 μl of the reverse primer (5 μM), 5 μl of the Gotaq ® Colorless Master Mix (Promega; 2X), and 10 μl of ultrapure water (Don et al., ; Korbie & Mattick, ).

Article Title: Genome-Assisted Gene-Flow Rescued Genetic Diversity Without Hindering Growth Performance in an Inbred Coho Salmon ( Oncorhynchus kisutch ) Population Selected for High Growth Phenotype
Article Snippet: Genomic DNA was collected from an adipose fin clip using a Gentra Puregene Tissue Kit (QIAGEN). .. The 11 loci were amplified using the tailed primer method (Schuelke ; Sekino et al. ) where 19-bp of the M13 sequence (CACGACGTTGTAAAACGAC) was added to the 5' end of the forward primer to label PCR amplicon with a fluorescent dye (i.e., FAM, VIC, NED, PED). .. Polymorphisms were analyzed on an ABI3130 DNA Analyzer (Applied Biosystems, MA, USA).

Article Title: The first reproductive parameters and evidence of multiple paternity in one new spiny dogfish species, Squalus albicaudus (Squaliformes, Squalidae).
Article Snippet: Understanding elasmobranch reproductive biology is necessary for species conservation.. Multiple paternity has been reported for elasmobranchs and here we investigate the reproductive aspects and mating system (paternity genetic analyses) for Squalus albicaudus.. Thirteen pregnant females were analyzed concerning reproductive parameters and mating system was assessed of nine females and their litters.

Sequencing:

Article Title: Genome-Assisted Gene-Flow Rescued Genetic Diversity Without Hindering Growth Performance in an Inbred Coho Salmon ( Oncorhynchus kisutch ) Population Selected for High Growth Phenotype
Article Snippet: Genomic DNA was collected from an adipose fin clip using a Gentra Puregene Tissue Kit (QIAGEN). .. The 11 loci were amplified using the tailed primer method (Schuelke ; Sekino et al. ) where 19-bp of the M13 sequence (CACGACGTTGTAAAACGAC) was added to the 5' end of the forward primer to label PCR amplicon with a fluorescent dye (i.e., FAM, VIC, NED, PED). .. Polymorphisms were analyzed on an ABI3130 DNA Analyzer (Applied Biosystems, MA, USA).



Similar Products

96
Addgene inc crispr cas9 genomic engineering idt star methods rbns primers idt star methods recombinant dna px458 addgene plasmid
Crispr Cas9 Genomic Engineering Idt Star Methods Rbns Primers Idt Star Methods Recombinant Dna Px458 Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+method/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/pm41932309-792-83-97
Average 96 stars, based on 1 article reviews
crispr cas9 genomic engineering idt star methods rbns primers idt star methods recombinant dna px458 addgene plasmid - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

90
Takeda methods primers
Methods Primers, supplied by Takeda, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+method/methods+primers/pm40613602-505-1-8
Average 90 stars, based on 1 article reviews
methods primers - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Sangon Biotech downstream and rt primers of the stem-loop method
Downstream And Rt Primers Of The Stem Loop Method, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+method/stem+loop+primers/pm40327646-110-7-13
Average 90 stars, based on 1 article reviews
downstream and rt primers of the stem-loop method - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Illumina Inc primers used for the illumina miseq and nanopore metabarcoding method
Primers Used For The Illumina Miseq And Nanopore Metabarcoding Method, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+method/primers+used+for+the+illumina+miseq+and+nanopore+metabarcoding+method/pm40247828-77-0-4
Average 90 stars, based on 1 article reviews
primers used for the illumina miseq and nanopore metabarcoding method - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
PrimerDesign Inc primers for the sslamp method targeting the sarscov-2 s gene
Primers For The Sslamp Method Targeting The Sarscov 2 S Gene, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+method/primers+for+the+sslamp+method+targeting+the+sarscov+2+s+gene/pm40167300-275-11-0
Average 90 stars, based on 1 article reviews
primers for the sslamp method targeting the sarscov-2 s gene - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Schuelke tailed primer method
Tailed Primer Method, supplied by Schuelke, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+method/tailed+primer+method/pmc11785656-117-7-10
Average 90 stars, based on 1 article reviews
tailed primer method - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Caredx Inc pcr sequencespecific primer method (pcrssp)
Pcr Sequencespecific Primer Method (Pcrssp), supplied by Caredx Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+method/pcr+sequence+specific+priming/pm39865484-6-1-9
Average 90 stars, based on 1 article reviews
pcr sequencespecific primer method (pcrssp) - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Caredx Inc pcr sequence- specific primer method (pcr- ssp)
Pcr Sequence Specific Primer Method (Pcr Ssp), supplied by Caredx Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+method/pcr+sequence+specific+priming/pm39865484-23-1-11
Average 90 stars, based on 1 article reviews
pcr sequence- specific primer method (pcr- ssp) - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Eurofins in-house method similar to the artic primers
In House Method Similar To The Artic Primers, supplied by Eurofins, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+method/in+house+method+similar+to+the+artic+primers/pm39705295-48-17-7
Average 90 stars, based on 1 article reviews
in-house method similar to the artic primers - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Thermo Fisher pcr primers for both semi-quantitative and quantitative methods
List of tested (white fields) and reference genes.
Pcr Primers For Both Semi Quantitative And Quantitative Methods, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+method/pmc11641612-201-0-15
Average 90 stars, based on 1 article reviews
pcr primers for both semi-quantitative and quantitative methods - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


List of tested (white fields) and reference genes.

Journal: International Journal of Molecular Sciences

Article Title: Biosynthesis of Phenolic Compounds of Medicago truncatula After Inoculation with Selected PGPR Strains

doi: 10.3390/ijms252312684

Figure Lengend Snippet: List of tested (white fields) and reference genes.

Article Snippet: PCR primers for both semi-quantitative and quantitative methods were designed using Primer Express 3.0 software (Applied Biosystems, Carlsbad, CA, USA)— .

Techniques: Sequencing